Home > Sandwich ELISA Kits > Human S100 Calcium Binding Protein A12 (S100A12) ELISA Kit

Product Name:

Human S100 Calcium Binding Protein A12 (S100A12) ELISA Kit

Cat.#:

JL19155

Regulatory Status:

RUO

Alternative:

CGRP; P6; CAAF1; CAGC; ENRAGE; MRP6; Calgranulin C; Calcium-binding protein in amniotic fluid 1; Extracellular newly identified RAGE-binding; Migration inhibitory factor-related 6

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.15-10ng/mL

Sensitivity:

0.08ng/mL

Specificity:

This kit recognizes Human S100A12 in samples. No significant cross-reactivity or interference between Human S100A12 and analogues was observed.

Sample Type:

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Cancer

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| S100A12

S100 calcium-binding protein A12 (S100A12) is a protein that in humans is encoded by the S100A12 gene.Human S100A12, also known as calgranulin C.The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs.Generally, S100A12 has a significant anti-infectious and antibacterial role that is related to its ability to uptake ions.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	10	5	2.5	1.25	0.62	0.31	0.15	0
OD	2.26	1.54	1.04	0.73	0.43	0.3	0.2	0.05
Corrected OD	2.21	1.49	0.99	0.68	0.38	0.25	0.15	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human S100A12 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Human S100A12 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-115
1 : 4	Average (%)	95	98	108